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M.S.GEL
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Cation Exchanger for Ion Exchange Chromatography
CM type：Carboxymethyl modified silica gel
SP type：Sulfopropyl modified silica gel
Characteristics
I

II


III		 .

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.		 Supporting MSGel does not swell on exposure to organic solvents like resin packing agent.
These are spherical particles (MSGEL), so it is possible to obtain low back pressure even with high flow rates. Thus, the columns can be scaled up and/or run with high flow rates to increase productivity.
Recovery rates during purification can be greatly improved by selecting the best porosity properties from the wide selection available in the MS Gel products.
Below shows the chromatographs of enzymes in various modified end-units and pore diameter.
Column:4.6×80mm, Eluent：A）20mM phosphate buffer (pH6.5),B) 20mM phosphate buffer containing 0.5M NaCl, 0-100%B(30min,linear), Flow rate:1.0mL/min, Detection:UV280nm at 30
1:Ribonuclease A(14kDa,pI=9.3)　2:α-chymotrypsinogen(25kDa,pI=9.5) 3:Lysozyme(14kDa,pI=10.7)
 
IV . EP (extra pure) series can separate materials without the influence of metal contamination.
(example)
Below shows the separation of the bioactivation amino compounds.
SP-EP-DF-15-60A can separate the compounds.
In contrast, SP-DF-15-60A has sufficient metal contamination resulting in absorption of the materials.
Column:4.6×250mm, Eluent：200mM CH3COONa + 20mM CH3COOH, Flow rate:1.0mL/min, Detection:UV254nm at 40
1.Noradrenalin 2.Octopamine 3.Dopamine 4.Tyramine
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